Genome-Scale Analysis of Metazoan Replication Origins Reveals their Organization in Specific but Flexible Sites Defined by Conserved Features

Abstract : In metazoans, thousands of DNA replication origins (Oris) are activated at each cell cycle. Their genomic organization and their genetic nature remain elusive. Here, we characterized Oris by nascent strand (NS) purification and a genome-wide analysis in Drosophila and mouse cells. We show that in both species most CpG islands (CGI) contain Oris, although methylation is nearly absent in Drosophila, indicating that this epigenetic mark is not crucial for defining the activated origin. Initiation of DNA synthesis starts at the borders of CGI, resulting in a striking bimodal distribution of NS, suggestive of a dual initiation event. Oris contain a unique nucleotide skew around NS peaks, characterized by G/T and C/A overrepresentation at the 5′ and 3′ of Ori sites, respectively. Repeated GC-rich elements were detected, which are good predictors of Oris, suggesting that common sequence features are part of metazoan Oris. In the heterochromatic chromosome 4 of Drosophila, Oris correlated with HP1 binding sites. At the chromosome level, regions rich in Oris are early replicating, whereas Ori-poor regions are late replicating. The genome-wide analysis was coupled with a DNA combing analysis to unravel the organization of Oris. The results indicate that Oris are in a large excess, but their activation does not occur at random. They are organized in groups of site-specific but flexible origins that define replicons, where a single origin is activated in each replicon. This organization provides both site specificity and Ori firing flexibility in each replicon, allowing possible adaptation to environmental cues and cell fates.
Liste complète des métadonnées

Littérature citée [13 références]  Voir  Masquer  Télécharger

https://hal-lirmm.ccsd.cnrs.fr/lirmm-00631491
Contributeur : Eric Rivals <>
Soumis le : jeudi 27 octobre 2011 - 07:00:19
Dernière modification le : jeudi 11 janvier 2018 - 06:26:13
Document(s) archivé(s) le : dimanche 4 décembre 2016 - 09:10:49

Fichiers

Supplementary_Figures.pdf
Fichiers produits par l'(les) auteur(s)

Identifiants

Collections

Citation

Christelle Cayrou, Philippe Coulombe, Alice Vigneron, Slavica Stanojcic, Olivier Ganier, et al.. Genome-Scale Analysis of Metazoan Replication Origins Reveals their Organization in Specific but Flexible Sites Defined by Conserved Features. Genome Research, Cold Spring Harbor Laboratory Press, 2011, 21 (9), pp.1438-1449. 〈http://genome.cshlp.org/content/21/9/1438.abstract〉. 〈10.1101/gr.121830.111〉. 〈lirmm-00631491〉

Partager

Métriques

Consultations de la notice

433

Téléchargements de fichiers

358