Minisatellite Markers Reveals Frequent Genetic Exchanges among House Mouse Subspecies

Eric Rivals 1
1 MAB - Méthodes et Algorithmes pour la Bioinformatique
LIRMM - Laboratoire d'Informatique de Robotique et de Microélectronique de Montpellier
Abstract : Background: Four hypervariable minisatellite loci were scored on a panel of 116 individuals of various geographical origins representing a large part of the diversity present in House Mouse subspecies. Internal structures of alleles were determined by Minisatellite Variant Repeat mapping PCR (MVR-PCR), to produce maps of intermingled patterns of variant repeats along the repeat array. To reconstruct the genealogy of these arrays of variable length, the specifically designed software MS_Align was used to estimate molecular divergences, graphically represented as neighbour-joining trees. Results: Given the high haplotypic diversity detected (mean He=0.962), these minisatellite trees proved to be highly informative for tracing past and present genetic exchanges. Examples of identical or nearly identical alleles were found across subspecies and in geographically very distant locations, together with a poor lineage sorting among subspecies except for the X chromosome locus MMS30 in M. m. musculus. Given the high mutation rate of mouse minisatellite loci, this picture cannot be interpreted only with simple splitting events followed by retention of polymorphism, but implies recurrent gene flow between already differentiated entities. Conclusion: This strongly suggests that, at least for the chromosomal regions under scrutiny, wild House Mice subspecies constitute a set of interrelated gene pools still connected through long range gene flow or genetic exchanges occurring in the various contact zones existing nowadays or having existed in the past. Identifying genomic regions that do not follow this pattern will be a challenging task for pinpointing genes important for speciation.
Type de document :
Communication dans un congrès
L. Duret; N. Galtier. Rencontres ALPHY - Génomique Evolutive, Bioinformatique, Alignement et Phylogénie, Feb 2007, Montpellier, France. pp.1, 2007
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https://hal-lirmm.ccsd.cnrs.fr/lirmm-00195524
Contributeur : Eric Rivals <>
Soumis le : mardi 11 décembre 2007 - 10:28:58
Dernière modification le : jeudi 11 janvier 2018 - 06:26:12
Document(s) archivé(s) le : jeudi 27 septembre 2012 - 11:11:16

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  • HAL Id : lirmm-00195524, version 1

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Eric Rivals. Minisatellite Markers Reveals Frequent Genetic Exchanges among House Mouse Subspecies. L. Duret; N. Galtier. Rencontres ALPHY - Génomique Evolutive, Bioinformatique, Alignement et Phylogénie, Feb 2007, Montpellier, France. pp.1, 2007. 〈lirmm-00195524〉

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