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Sequencing of the smallest apicomplexan genome from the human pathogen Babesia Microti

Emmanuel Cornillot 1, * Kamel Hadj-Kaddour 1 Amina Dassouli 1 Benjamin Noel 2 Vincent Ranwez 3 Benoit Vacherie 4 Yoann Augagneur 5 Virginie Brès 1 Aurelie Duclos 2 Sylvie Randazzo 1 Bernard Carcy 1 Françoise Debierre-Grockiego 6 Stéphane Delbecq 7 Karina Moubri-Ménage 1 Hosam Shams-Eldin 8 Sahar Usmani-Brown 5 Frédéric Bringaud 9 Patrick Wincker 4 Christian Vivarès 1 Ralph T. Schwarz 8 Theo P. Schetters 10 Peter J. Krause 5 André Gorenflot 11 Vincent Berry 12 Valérie Barbe 4 Choukri Ben Mamoun 5
* Corresponding author
1 LMGE - Laboratoire Microorganismes : Génome et Environnement
2 UMR 8030 - Génomique métabolique
3 UMR AGAP - Amélioration génétique et adaptation des plantes méditerranéennes et tropicales
4 GENOSCOPE - Genoscope - Centre national de séquençage [Evry]
5 Department of Internal Medecine - Department of Internal Medecine, Yale
6 ISP-311 - Infectiologie Santé Publique
7 LBCM - Vaccination Antiparasitaire : Laboratoire de Biologie Cellulaire et Moléculaire
8 Institut für Virologie
9 RMSB - Résonance magnétique des systèmes biologiques
10 Microbiology R&D Department - Microbiology R&D Department, Intervet/Schering-Plough Animal Health
11 DIMNP - Dynamique des interactions membranaires normales et pathologiques
12 MAB - Méthodes et Algorithmes pour la Bioinformatique
LIRMM - Laboratoire d'Informatique de Robotique et de Microélectronique de Montpellier
Abstract : We have sequenced the genome of the emerging human pathogen Babesia microti and compared it with that of other protozoa. B. microti has the smallest nuclear genome among all Apicomplexan parasites sequenced to date with three chromosomes encoding ∼3500 polypeptides, several of which are species specific. Genome-wide phylogenetic analyses indicate that B. microti is significantly distant from all species of Babesidae and Theileridae and defines a new clade in the phylum Apicomplexa. Furthermore, unlike all other Apicomplexa, its mitochondrial genome is circular. Genome-scale reconstruction of functional networks revealed that B. microti has the minimal metabolic requirement for intraerythrocytic protozoan parasitism. B. microti multigene families differ from those of other protozoa in both the copy number and organization. Two lateral transfer events with significant metabolic implications occurred during the evolution of this parasite. The genomic sequencing of B. microti identified several targets suitable for the development of diagnostic assays and novel therapies for human babesiosis.
Keywords : Babesia microty eukaryote genome
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CEA | CNRS | UNIV-EVRY | LMGE | PRES_CLERMONT | CIRAD | INRA | MAB | ACL-SVSAE | AGROPOLIS | LIRMM | GENOMIQUE-METABOLIQUE | MIPS | BS | UNIV-MONTPELLIER | JACOB | CEA-DRF | UNIV-TOURS | GENOSCOPE | MONTPELLIER-SUPAGRO | INRAE

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Emmanuel Cornillot, Kamel Hadj-Kaddour, Amina Dassouli, Benjamin Noel, Vincent Ranwez, et al.. Sequencing of the smallest apicomplexan genome from the human pathogen Babesia Microti. Nucleic Acids Research, Oxford University Press, 2012, 40 (18), pp.9102-9114. ⟨10.1093/nar/gks700⟩. ⟨lirmm-00818833⟩

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