PlasmoDraft: a database of Plasmodium falciparum gene function predictions based on postgenomic data

Laurent Brehelin 1, * Jean-François Dufayard 2 Olivier Gascuel 1
* Auteur correspondant
1 MAB - Méthodes et Algorithmes pour la Bioinformatique
LIRMM - Laboratoire d'Informatique de Robotique et de Microélectronique de Montpellier
Abstract : Background: Of the 5,484 predicted proteins of Plasmodium falciparum, the main causative agent of malaria, about 60\% do not have sufficient sequence similarity with proteins in other organisms to warrant provision of functional assignments. Non-homology methods are thus needed to obtain functional clues for these uncharacterized genes. Method: We present PlasmoDraft, a database of Gene Ontology (GO) annotation predictions for P. falciparum genes based on postgenomic data. Predictions of PlasmoDraft are achieved with a "Guilt By Association" method named Gonna. This involves (1) a predictor that proposes GO annotations for a gene based on the similarity of its profile (measured with transcriptome, proteome or interactome data) with genes already annotated by GeneDB; (2) a procedure that estimates the confidence of the predictions achieved with each data source; (3) a procedure that combines all data sources to provide a global summary and confidence estimate of the predictions. Results: Gonna has been applied to all P. falciparum genes using most publicly available transcriptome, proteome and interactome data sources. Gonna provides predictions for numerous genes without any annotations. For example, 2,434 genes without any annotations in the Biological Process ontology are associated with specific GO terms (e.g. Rosetting, Antigenic variation), and among these, 841 have confidence values above 50%. In the Cellular Component and Molecular Function ontologies, 1,905 and 1,540 uncharacterized genes are associated with specific GO terms, respectively (740 and 329 with confidence value above 50\%). All predictions along with their confidence values have been compiled in PlasmoDraft, which thus provides an extensive database of GO annotation predictions that can be achieved with these data sources. The database can be accessed in different ways. A global view allows for a quick inspection of the GO terms that are predicted with high confidence, depending on the various data sources. A gene view and a GO term view allow for the search of potential GO terms attached to a given gene, and genes that potentially belong to a given GO term.
Type de document :
Article dans une revue
BMC Bioinformatics, BioMed Central, 2008, 9, pp.9:440. 〈http://www.biomedcentral.com/1471-2105/9/440〉. 〈10.1186/1471-2105-9-440〉
Liste complète des métadonnées

Littérature citée [34 références]  Voir  Masquer  Télécharger

https://hal-lirmm.ccsd.cnrs.fr/lirmm-00327273
Contributeur : Isabelle Gouat <>
Soumis le : mercredi 5 septembre 2012 - 14:37:36
Dernière modification le : jeudi 11 janvier 2018 - 06:26:12
Document(s) archivé(s) le : vendredi 16 décembre 2016 - 09:55:42

Fichier

BrehelinDufayardGascuel_BMCBio...
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

Collections

Citation

Laurent Brehelin, Jean-François Dufayard, Olivier Gascuel. PlasmoDraft: a database of Plasmodium falciparum gene function predictions based on postgenomic data. BMC Bioinformatics, BioMed Central, 2008, 9, pp.9:440. 〈http://www.biomedcentral.com/1471-2105/9/440〉. 〈10.1186/1471-2105-9-440〉. 〈lirmm-00327273v2〉

Partager

Métriques

Consultations de la notice

154

Téléchargements de fichiers

176